Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Error in rule repair #116

Open
Eigertje opened this issue Nov 23, 2021 · 0 comments
Open

Error in rule repair #116

Eigertje opened this issue Nov 23, 2021 · 0 comments

Comments

@Eigertje
Copy link

I'm running DropSeqPipe v5.0 with 5 samples in my samples.csv file. However, the pipeline aborted on 1 sample during "rule repair".
After rerunning the pipeline with this sample removed the error occured again on another sample. For the other 3 samples the whole DropSeqPipe pipeline worked fine. Any idea what is happening here? I'm not familiar at all with python and snakemake I'm afraid.

The error:
`Error in rule repair:
jobid: 82
output: ../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, ../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz
log: ../Results/logs/bbmap/HN4_RNA_repair.txt (check log file(s) for error message)
conda-env: /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964
shell:
repair.sh -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4 2> ../Results/logs/bbmap/HN4_RNA_repair.txt
(one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)

Removing output files of failed job repair since they might be corrupted:
../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, ../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz
`

The content of the "/mnt/go/temp/scRNA-seq/Results/logs/bbmap/HN4_RNA_repair.txt" file:
`java -ea -Xmx20g -cp /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/opt/bbmap-38.22-1/current/ jgi.SplitPairsAndSingles rp -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4
Executing jgi.SplitPairsAndSingles [rp, -Xmx20g, in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz, in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz, out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz, out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz, repair=t, threads=4]

Set threads to 4
Set INTERLEAVED to false
Started output stream.
/mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/bin/repair.sh: line 104: 18758 Killed java -ea -Xmx20g -cp /mnt/go/temp/scRNA-seq/dropSeqPipe/.snakemake/conda/65b8583675236a3c6fd8572f06a27964/opt/bbmap-38.22-1/current/ jgi.SplitPairsAndSingles rp -Xmx20g in=../Results/samples/HN4_RNA/trimmed_R1.fastq.gz in2=../Results/samples/HN4_RNA/trimmed_R2.fastq.gz out1=../Results/samples/HN4_RNA/trimmed_repaired_R1.fastq.gz out2=../Results/samples/HN4_RNA/trimmed_repaired_R2.fastq.gz repair=t threads=4
`

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant