Error computing FDR: Cannot compute FDR Scores; target file is empty

[Arrow-V]

I directly use MSPathFinderT to analyze my data(Thermo .raw), however, it goes wrong. I don't know know how to fix it, could anybody help me?

C:\Users\Neal\Documents>MSPathFinderT.exe -s D:\10_26data\Chem2-2-1.raw -d D:\10_26data\all.fasta -o D:\10_26data\new -t 10 -f 10 -m 1 -tda 1 -minLength 21 -maxLength 300 -minCharge 2 -maxCharge 30 -minFragCharge 1 -maxFragCharge 15 -minMass 3000 -maxMass 50000 -mod D:\10_26data\MSPathFinder_Mods.txt
MSPathFinderT version 1.0.6510 (Oct. 28, 2017)
MaxThreads: 6
SpectrumFilePath: D:\10_26data\Chem2-2-1.raw
DatabaseFilePath: D:\10_26data\all.fasta
FeatureFilePath: N/A
OutputDir: D:\10_26data\new
InternalCleavageMode: SingleInternalCleavage
Tag-based search: True
Tda: Target+Decoy
PrecursorIonTolerancePpm: 10
ProductIonTolerancePpm: 10
MinSequenceLength: 21
MaxSequenceLength: 300
MinPrecursorIonCharge: 2
MaxPrecursorIonCharge: 30
MinProductIonCharge: 1
MaxProductIonCharge: 15
MinSequenceMass: 3000
MaxSequenceMass: 50000
MaxDynamicModificationsPerSequence: 4
Modifications:
C(0) H(0) N(0) O(1) S(0),M,opt,Everywhere,Oxidation
C(0) H(-1) N(0) O(0) S(0),C,opt,Everywhere,Dehydro
C(2) H(2) N(0) O(1) S(0),*,opt,ProteinNTerm,Acetyl
Creating and loading pbf file...
Total Progress: 0.00%, 0d 0h 0.00m elapsed, Current Task: Reading spectra file
Elapsed Time: 5.2 sec
Reading Fasta File
Generating D:\10_26data\all.icseq and
Generating D:\10_26data\all.icanno ... Done
Reading ProMex results...
3155/3155 features loaded...Elapsed Time: 2.0 sec
Generating deconvoluted spectra for MS/MS spectra...
Elapsed Time: 0.0 sec
Generating sequence tags for MS/MS spectra...
Number of spectra: 0
Generated sequence tags: 0
Elapsed Time: 0.0 sec
Caching peaks in MS1 spectra: 25329 scans
Sorting MS1 peaks: 54,689,404 peaks
Reading the target database...
Elapsed Time: 0.3 sec
Tag-based searching the target database
Number of spectra containing sequence tags: 0
Collected candidate matches: 0
Target database tag-based search elapsed Time: 35.5 sec
Searching the target database
Generating D:\10_26data\all.icplcp ... Done
Estimated Sequences: 37,834,838
Processing, 0 proteins done, 0.0% complete, 0.0 sec elapsed
Processing, 106315 proteins done, 0.3% complete, 15.1 sec elapsed
Processing, 118028 proteins done, 0.3% complete, 30.1 sec elapsed
Processing, 216527 proteins done, 0.6% complete, 45.1 sec elapsed
Processing, 323031 proteins done, 0.9% complete, 60.1 sec elapsed
Processing, 427459 proteins done, 1.1% complete, 75.1 sec elapsed
Total Progress: 42.70%, 0d 0h 5.00m elapsed, Current Task: Searching the target database
Processing, 532183 proteins done, 1.4% complete, 90.1 sec elapsed
Processing, 637148 proteins done, 1.7% complete, 105.1 sec elapsed
Processing, 768883 proteins done, 2.0% complete, 135.1 sec elapsed
Processing, 953651 proteins done, 2.5% complete, 165.1 sec elapsed
Processing, 1101862 proteins done, 2.9% complete, 195.9 sec elapsed
Processing, 1285452 proteins done, 3.4% complete, 225.9 sec elapsed
Processing, 1422493 proteins done, 3.8% complete, 255.9 sec elapsed
Processing, 1510383 proteins done, 4.0% complete, 285.9 sec elapsed
Processing, 1672899 proteins done, 4.4% complete, 346.0 sec elapsed
Total Progress: 43.36%, 0d 0h 10.00m elapsed, Current Task: Searching the target database
Processing, 2041036 proteins done, 5.4% complete, 406.0 sec elapsed
Processing, 2368429 proteins done, 6.3% complete, 466.0 sec elapsed
Processing, 2683408 proteins done, 7.1% complete, 527.5 sec elapsed
Processing, 2991146 proteins done, 7.9% complete, 587.5 sec elapsed
Processing, 3309509 proteins done, 8.7% complete, 647.5 sec elapsed
Total Progress: 44.12%, 0d 0h 15.04m elapsed, Current Task: Searching the target database
Processing, 3681335 proteins done, 9.7% complete, 707.5 sec elapsed
Processing, 4036809 proteins done, 10.7% complete, 767.5 sec elapsed
Processing, 4308919 proteins done, 11.4% complete, 827.6 sec elapsed
Processing, 4548002 proteins done, 12.0% complete, 887.8 sec elapsed
Processing, 4752700 proteins done, 12.6% complete, 947.8 sec elapsed
Total Progress: 44.75%, 0d 0h 20.09m elapsed, Current Task: Searching the target database
Processing, 4939771 proteins done, 13.1% complete, 1007.8 sec elapsed
Processing, 4939772 proteins done, 13.1% complete, 1007.8 sec elapsed
Processing, 4939773 proteins done, 13.1% complete, 1007.8 sec elapsed
Processing, 5122628 proteins done, 13.5% complete, 1069.0 sec elapsed
Processing, 5122627 proteins done, 13.5% complete, 1069.0 sec elapsed
Processing, 5328186 proteins done, 14.1% complete, 1129.0 sec elapsed
Processing, 5535302 proteins done, 14.6% complete, 1191.3 sec elapsed
Total Progress: 45.21%, 0d 0h 25.09m elapsed, Current Task: Searching the target database
Processing, 6754622 proteins done, 17.9% complete, 1492.6 sec elapsed
Total Progress: 45.77%, 0d 0h 30.09m elapsed, Current Task: Searching the target database
Processing, 7896082 proteins done, 20.9% complete, 1792.6 sec elapsed
Total Progress: 46.38%, 0d 0h 35.14m elapsed, Current Task: Searching the target database
Processing, 9470041 proteins done, 25.0% complete, 2092.6 sec elapsed
Total Progress: 47.03%, 0d 0h 40.14m elapsed, Current Task: Searching the target database
Processing, 10584900 proteins done, 28.0% complete, 2393.7 sec elapsed
Total Progress: 47.50%, 0d 0h 45.19m elapsed, Current Task: Searching the target database
Processing, 11487978 proteins done, 30.4% complete, 2694.0 sec elapsed
Total Progress: 47.98%, 0d 0h 50.21m elapsed, Current Task: Searching the target database
Processing, 12217755 proteins done, 32.3% complete, 2994.0 sec elapsed
Total Progress: 48.23%, 0d 0h 55.22m elapsed, Current Task: Searching the target database
Processing, 12725499 proteins done, 33.6% complete, 3296.6 sec elapsed
Total Progress: 48.39%, 0d 1h 0.26m elapsed, Current Task: Searching the target database
Total Progress: 48.39%, 0d 1h 5.27m elapsed, Current Task: Searching the target database
Total Progress: 48.39%, 0d 1h 10.29m elapsed, Current Task: Searching the target database
Total Progress: 48.39%, 0d 1h 15.31m elapsed, Current Task: Searching the target database
Total Progress: 48.39%, 0d 1h 20.32m elapsed, Current Task: Searching the target database
Total Progress: 48.39%, 0d 1h 25.34m elapsed, Current Task: Searching the target database
Processing, 12725500 proteins done, 33.6% complete, 5000.6 sec elapsed
Total Progress: 48.84%, 0d 1h 30.34m elapsed, Current Task: Searching the target database
Processing, 14227281 proteins done, 37.6% complete, 5300.6 sec elapsed
Total Progress: 49.45%, 0d 1h 35.38m elapsed, Current Task: Searching the target database
Processing, 15403529 proteins done, 40.7% complete, 5600.7 sec elapsed
Total Progress: 49.87%, 0d 1h 40.38m elapsed, Current Task: Searching the target database
Processing, 16221767 proteins done, 42.9% complete, 5900.7 sec elapsed
Total Progress: 50.28%, 0d 1h 45.39m elapsed, Current Task: Searching the target database
Processing, 17104889 proteins done, 45.2% complete, 6203.2 sec elapsed
Total Progress: 50.69%, 0d 1h 50.39m elapsed, Current Task: Searching the target database
Processing, 18226901 proteins done, 48.2% complete, 6505.3 sec elapsed
Total Progress: 51.20%, 0d 1h 55.39m elapsed, Current Task: Searching the target database
Processing, 19098473 proteins done, 50.5% complete, 6806.7 sec elapsed
Total Progress: 51.62%, 0d 2h 0.42m elapsed, Current Task: Searching the target database
Processing, 20256051 proteins done, 53.5% complete, 7106.7 sec elapsed
Total Progress: 52.17%, 0d 2h 5.42m elapsed, Current Task: Searching the target database
Processing, 21211621 proteins done, 56.1% complete, 7406.7 sec elapsed
Total Progress: 52.57%, 0d 2h 10.43m elapsed, Current Task: Searching the target database
Processing, 22091816 proteins done, 58.4% complete, 7708.7 sec elapsed
Total Progress: 53.12%, 0d 2h 15.48m elapsed, Current Task: Searching the target database
Processing, 23624794 proteins done, 62.4% complete, 8008.7 sec elapsed
Total Progress: 53.93%, 0d 2h 20.48m elapsed, Current Task: Searching the target database
Processing, 25145852 proteins done, 66.5% complete, 8311.5 sec elapsed
Total Progress: 54.50%, 0d 2h 25.51m elapsed, Current Task: Searching the target database
Processing, 26387047 proteins done, 69.7% complete, 8613.3 sec elapsed
Total Progress: 55.08%, 0d 2h 30.54m elapsed, Current Task: Searching the target database
Processing, 27652102 proteins done, 73.1% complete, 8913.3 sec elapsed
Total Progress: 55.66%, 0d 2h 35.54m elapsed, Current Task: Searching the target database
Processing, 28895951 proteins done, 76.4% complete, 9215.1 sec elapsed
Total Progress: 56.24%, 0d 2h 40.58m elapsed, Current Task: Searching the target database
Processing, 30139855 proteins done, 79.7% complete, 9515.2 sec elapsed
Total Progress: 56.80%, 0d 2h 45.59m elapsed, Current Task: Searching the target database
Processing, 31342528 proteins done, 82.8% complete, 9815.2 sec elapsed
Total Progress: 57.34%, 0d 2h 50.59m elapsed, Current Task: Searching the target database
Processing, 32435944 proteins done, 85.7% complete, 10117.8 sec elapsed
Total Progress: 57.87%, 0d 2h 55.59m elapsed, Current Task: Searching the target database
Processing, 33930982 proteins done, 89.7% complete, 10417.8 sec elapsed
Total Progress: 58.68%, 0d 3h 0.61m elapsed, Current Task: Searching the target database
Processing, 35360872 proteins done, 93.5% complete, 10717.8 sec elapsed
Total Progress: 59.19%, 0d 3h 5.62m elapsed, Current Task: Searching the target database
Processing, 36518054 proteins done, 96.5% complete, 11017.8 sec elapsed
Total Progress: 59.74%, 0d 3h 10.67m elapsed, Current Task: Searching the target database
Processing, 37390107 proteins done, 98.8% complete, 11319.5 sec elapsed
Collected candidate matches: 0
Target database search elapsed Time: 11399.8 sec
Calculating spectral E-values for target-spectrum matches
Estimated matched sequences: 0
Target-spectrum match E-value calculation elapsed Time: 0.1 sec
Creating D:\10_26data\all.icsfldecoy.fasta
Generating D:\10_26data\all.icsfldecoy.icseq and
Generating D:\10_26data\all.icsfldecoy.icanno ... Done
Reading the decoy database...
Elapsed Time: 0.5 sec
Tag-based searching the decoy database
Number of spectra containing sequence tags: 0
Collected candidate matches: 0
Decoy database tag-based search elapsed Time: 12.1 sec
Searching the decoy database
Generating D:\10_26data\all.icsfldecoy.icplcp ... Done
Estimated Sequences: 37,834,838
Processing, 0 proteins done, 0.0% complete, 0.0 sec elapsed
Processing, 91058 proteins done, 0.2% complete, 15.0 sec elapsed
Processing, 104777 proteins done, 0.3% complete, 36.5 sec elapsed
Processing, 104775 proteins done, 0.3% complete, 36.5 sec elapsed
Processing, 104780 proteins done, 0.3% complete, 36.5 sec elapsed
Processing, 199050 proteins done, 0.5% complete, 51.5 sec elapsed
Processing, 299404 proteins done, 0.8% complete, 66.5 sec elapsed
Processing, 399679 proteins done, 1.1% complete, 81.6 sec elapsed
Processing, 473324 proteins done, 1.3% complete, 99.3 sec elapsed
Total Progress: 77.72%, 0d 3h 15.70m elapsed, Current Task: Searching the decoy database
Processing, 473827 proteins done, 1.3% complete, 116.2 sec elapsed
Processing, 473826 proteins done, 1.3% complete, 116.2 sec elapsed
Processing, 473828 proteins done, 1.3% complete, 116.2 sec elapsed
Processing, 474920 proteins done, 1.3% complete, 147.2 sec elapsed
Processing, 576058 proteins done, 1.5% complete, 177.2 sec elapsed
Processing, 776854 proteins done, 2.1% complete, 207.2 sec elapsed
Processing, 978487 proteins done, 2.6% complete, 237.2 sec elapsed
Processing, 1122059 proteins done, 3.0% complete, 267.2 sec elapsed
Processing, 1322696 proteins done, 3.5% complete, 297.2 sec elapsed
Processing, 1707488 proteins done, 4.5% complete, 357.2 sec elapsed
Total Progress: 78.42%, 0d 3h 20.70m elapsed, Current Task: Searching the decoy database
Processing, 2026830 proteins done, 5.4% complete, 417.3 sec elapsed
Processing, 2367141 proteins done, 6.3% complete, 478.8 sec elapsed
Processing, 2721653 proteins done, 7.2% complete, 538.8 sec elapsed
Processing, 3082872 proteins done, 8.1% complete, 598.8 sec elapsed
Processing, 3434130 proteins done, 9.1% complete, 658.8 sec elapsed
Total Progress: 79.22%, 0d 3h 25.70m elapsed, Current Task: Searching the decoy database
Processing, 3745457 proteins done, 9.9% complete, 718.8 sec elapsed
Processing, 3996486 proteins done, 10.6% complete, 778.8 sec elapsed
Processing, 4350716 proteins done, 11.5% complete, 838.9 sec elapsed
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Processing, 4716229 proteins done, 12.5% complete, 904.6 sec elapsed
Processing, 5132499 proteins done, 13.6% complete, 964.6 sec elapsed
Total Progress: 80.00%, 0d 3h 30.71m elapsed, Current Task: Searching the decoy database
Processing, 5520902 proteins done, 14.6% complete, 1024.6 sec elapsed
Processing, 6057630 proteins done, 16.0% complete, 1084.6 sec elapsed
Processing, 6560208 proteins done, 17.3% complete, 1144.6 sec elapsed
Total Progress: 81.24%, 0d 3h 35.71m elapsed, Current Task: Searching the decoy database
Processing, 9229408 proteins done, 24.4% complete, 1444.6 sec elapsed
Total Progress: 82.43%, 0d 3h 40.71m elapsed, Current Task: Searching the decoy database
Processing, 11711076 proteins done, 31.0% complete, 1744.7 sec elapsed
Total Progress: 83.50%, 0d 3h 45.71m elapsed, Current Task: Searching the decoy database
Processing, 13778610 proteins done, 36.4% complete, 2044.7 sec elapsed
Total Progress: 84.34%, 0d 3h 50.71m elapsed, Current Task: Searching the decoy database
Processing, 15581306 proteins done, 41.2% complete, 2344.7 sec elapsed
Total Progress: 85.13%, 0d 3h 55.71m elapsed, Current Task: Searching the decoy database
Processing, 17190416 proteins done, 45.4% complete, 2644.7 sec elapsed
Total Progress: 85.85%, 0d 4h 0.71m elapsed, Current Task: Searching the decoy database
Processing, 18745544 proteins done, 49.5% complete, 2944.7 sec elapsed
Total Progress: 86.40%, 0d 4h 5.71m elapsed, Current Task: Searching the decoy database
Processing, 19900230 proteins done, 52.6% complete, 3244.7 sec elapsed
Total Progress: 87.10%, 0d 4h 10.71m elapsed, Current Task: Searching the decoy database
Processing, 21381784 proteins done, 56.5% complete, 3546.7 sec elapsed
Total Progress: 87.72%, 0d 4h 15.76m elapsed, Current Task: Searching the decoy database
Processing, 22653945 proteins done, 59.9% complete, 3846.7 sec elapsed
Total Progress: 88.33%, 0d 4h 20.76m elapsed, Current Task: Searching the decoy database
Processing, 24074766 proteins done, 63.6% complete, 4147.1 sec elapsed
Total Progress: 88.99%, 0d 4h 25.76m elapsed, Current Task: Searching the decoy database
Processing, 25388499 proteins done, 67.1% complete, 4447.1 sec elapsed
Total Progress: 89.56%, 0d 4h 30.81m elapsed, Current Task: Searching the decoy database
Processing, 26602039 proteins done, 70.3% complete, 4748.9 sec elapsed
Total Progress: 90.13%, 0d 4h 35.85m elapsed, Current Task: Searching the decoy database
Processing, 27836037 proteins done, 73.6% complete, 5050.5 sec elapsed
Total Progress: 90.70%, 0d 4h 40.86m elapsed, Current Task: Searching the decoy database
Processing, 29086254 proteins done, 76.9% complete, 5351.3 sec elapsed
Total Progress: 91.28%, 0d 4h 45.88m elapsed, Current Task: Searching the decoy database
Processing, 30335458 proteins done, 80.2% complete, 5651.3 sec elapsed
Total Progress: 91.84%, 0d 4h 50.88m elapsed, Current Task: Searching the decoy database
Processing, 31534228 proteins done, 83.3% complete, 5951.9 sec elapsed
Processing, 31534229 proteins done, 83.3% complete, 5951.9 sec elapsed
Total Progress: 92.36%, 0d 4h 55.91m elapsed, Current Task: Searching the decoy database
Processing, 32562446 proteins done, 86.1% complete, 6254.3 sec elapsed
Total Progress: 92.87%, 0d 5h 0.93m elapsed, Current Task: Searching the decoy database
Processing, 33775607 proteins done, 89.3% complete, 6557.1 sec elapsed
Total Progress: 93.44%, 0d 5h 5.98m elapsed, Current Task: Searching the decoy database
Processing, 34961566 proteins done, 92.4% complete, 6858.7 sec elapsed
Total Progress: 93.96%, 0d 5h 11.00m elapsed, Current Task: Searching the decoy database
Processing, 36073780 proteins done, 95.3% complete, 7160.8 sec elapsed
Total Progress: 94.51%, 0d 5h 16.05m elapsed, Current Task: Searching the decoy database
Processing, 37283875 proteins done, 98.5% complete, 7460.8 sec elapsed
Collected candidate matches: 0
Decoy database search elapsed Time: 7626.0 sec
Calculating spectral E-values for decoy-spectrum matches
Estimated matched sequences: 0
Decoy-spectrum match E-value calculation elapsed Time: 0.0 sec

Warning: Error computing FDR: Cannot compute FDR Scores; target file is empty
Error processing Chem2-2-1.raw: Cannot compute FDR Scores; target file is empty

[FarmGeek4Life]

The error is that you have no results for the target or the decoy search. The only part of the entire process that shows any results is ProMex, which reports only 3155 features.