construct_RNA_seq_coverage_file.py

### Boas Pucker ###
### bpucker@cebitec.uni-bielefeld.de ###
### v0.2 ###

__usage__ = """
					python construct_RNA_seq_coverage_file.py\n
					--in <BAM_FILE>
					--out <OUTPUT_FILE>
					
					--bam_is_sorted <PREVENTS_EXTRA_SORTING_OF_BAM_FILE>
					
					feature requests and bug reports: bpucker@cebitec.uni-bielefeld.de
					"""

import os, sys

# --- end of imports --- #

def main( arguments ):
	
	bam_file = arguments[ arguments.index( '--in' )+1 ]
	output_file = arguments[ arguments.index( '--out' )+1 ]
		
	samtools = "samtools"
	bedtools = "genomeCoverageBed"
	
	if '--bam_is_sorted' in arguments:
		sorted_bam_file = bam_file
	else:
		print "sorting BAM file ..."
		sorted_bam_file = output_file + "_sorted.bam"
		cmd = samtools + " sort -m 5000000000 --threads 8 " + bam_file + " > " + sorted_bam_file
		os.popen( cmd )
	
	# --- calculate read coverage depth per position --- #
	print "calculating coverage per position ...."
	cmd = bedtools + " -d -split -ibam " + sorted_bam_file + " > " + output_file
	os.popen( cmd )


if __name__ == '__main__':
	
	if '--in' in sys.argv and '--out' in sys.argv:
		main( sys.argv )
	else:
		sys.exit( __usage__ )
	
	print "all done!"