Tris recipe for buffering V. fischeri growth media.
Amount | Reagent |
---|---|
127 g | Tris-HCl |
23.6 g | Tris base |
Stir until dissolved, adjust to pH 7.5, then adjust volume to 1L with dI H2O.
Filter-sterilize and store at room temperature.
242 g Tris Base 57.1 mL Acetic acid, glacial 100 ml EDTA (0.5 M)
Adjust volume to 1L with dI H2O.
Store at room temperature.
ethylenediaminetetraacetic acid
Dissolve 186.1 g of disodium EDTA in 800 mL water.
Adjust pH with NaOH.
Adjust volume to 1L with dI H2O. Aliquot and autoclave.
Note: EDTA will not go into solution until the pH is approximately 8.0.
Add 250 mg of ethidium bromide to 25 mL of dH2O in a conical tube (amber, if available).
If not amber, wrap in aluminum foil. Invert on a wrist shaker for several hours to ensure that the dye has dissolved.
Store at room temperature.