This needs to be set up every time the instrument is powered on
- Turn on instrument.
- Select
Run User Protocol
. - Select desired protocl; e.g.
6 SEC-Std
.
The correct program is now set.
- Select
Show parameters
. - Select
OK
. - Select
Tools
. - Select
Settings
. - Select
Lid start is off
.
This toggles the lid start to on.
If dust is present in the instrument clean using a compressed air duster.
- Open lid.
- Insert sample.
- Close lid, allow reading to completely finish.
- For squid, open lid immediately after reading to prevent expulsion.
Troubleshooting: If the lid does not read on close, select Measure Luminescence
manually. Then reset lid start beginning above at the Show parameters
step (this commonly happens every 60-70 samples). Confirm that the correct protocol is still selected.
Record the reading (RLU) and the vessel (e.g., Drosophila vial or 1.5 ml microfuge tube with adapter).
Note: For luminescence reading of cultures, vortex for a precise time prior to reading so that samples are uniformly oxygenated. The vortex should be placed next to the luminometer and vortex time (e.g., 5 s) should be measured with a timer.
This only needs to be conducted once in the lifetime of the instrument (unless memory is reset, etc.).
Kinetics: OFF.
Frequency: 1/1 sec.
Integration 1: 6 sec, Total number of measurements: 10