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Single end reads error-No input file found, make sure it are fastq files No such file or directory #213

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dimserg opened this issue Oct 9, 2023 · 1 comment

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@dimserg
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dimserg commented Oct 9, 2023

Hello,

thank you for this valuable tool!
I am trying to run a chloroplast assembly using SE reads and I am getting this error every time:

No input file found, make sure it are fastq files No such file or directory

I am giving the directory of the single reads file in the "Forward reads" in the config.txt file. Here is my config.txt. Is there something I am missing because I am trying to use single end reads?

Thank you!

Project:

Project name = 311_chloro
Type = chloro
Genome Range = 120000-180000
K-mer = 39
Max memory = 8
Extended log =
Save assembled reads =
Seed Input = /home/projects/dimser/NovoPlasty/seed.fasta
Extend seed directly =
Reference sequence =
Variance detection =
Chloroplast sequence =

Dataset 1:

Read Length = 151
Insert size = 300
Platform = illumina
Single/Paired = SE
Combined reads =
Forward reads = /home/projects/dimser/NovoPlasty/single_reads/311_sigle.fastq
Reverse reads =
Store Hash =

Heteroplasmy:

MAF =
HP exclude list =
PCR-free =

Optional:

Insert size auto = yes
Use Quality Scores = no
Reduce ambigious N's =
Output path =

@ndierckx
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Hi,
If you use the SE mode you should put the fastq path under the combined reads.

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