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Minimum header requirements to be accepted as input? #223
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Hi, The reads you show here, are they each others pair, because the ids are not matching in any way.. |
Hello again! Reverse: I Did try to run it and got the following error (Log file is attached) : THE INPUT READS HAVE AN INCORRECT FILE FORMAT! One way I could make it work though was by changing the format of the file from fastq to fasta. All the best and thank you for taking the time to respond. |
Hello!
I'm trying to assemble the mitogenome for sequence data that has the following header format which seems to have been edited, any idea if I can modify it so that it's accepted as input?
Forwards:
@SRR17088602.9 9/1
GNATCCTTGGATAAATTTATCTACTATTGGTTATAAAAAAGTTATGATAGGGAAATTAAAAAATATCGGATATTAATTGACACTTAAGAACTTCAAAGTGATGTGGCATCTCGAATTCAAATCCGTTTTGCTATATTAATAACTCGTTAG
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF::F,:F:
@SRR17088602.10 10/1
TNATTGTTTCCCCTCCAAGATTAGAAAAGAGAATAAAAAGACATGTTCCTTCTTCACCACCACTACTACCAAAAGAGAGTCAAGTATAAATTATTTTTTTTCAAAAATATTTTAATTACTTTTCTTAGCTATCTTCGCCACCAAGAATAG
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFF::F::FFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF
Reverse:
@SRR17088602.4 4/2
GTTATGATAGAAAAATTAAAAAATATCGGATATTAATTGACACTTAAGAACTTCACAGTGATGTGGCATCTCGAATTCAAATCCGTTTTGAAATATTAATAGCTCGTTAGAATCAGCATGAAAAACTGTATTAGAATCCTTGGATAAATT
+
F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFF,FFFFFFFFFFFFFF,FFFFFFFFFF
@SRR17088602.5 5/2
GGAATGTTCTAAAGGATCTTTCAGTAACTGAATTTCCTTGAGACCAATGCGGTGTTGCATAGCTTTTGTTAATGTATAGCATTGAACAGAAATCCTTAAAGAACTCGCTTGTGAAAGCTGTGGCATTGTCAGTTACTAGCTCTACGCAGC
+
FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
All the best.
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