Minimum header requirements to be accepted as input?

[lightworms]

Hello!

I'm trying to assemble the mitogenome for sequence data that has the following header format which seems to have been edited, any idea if I can modify it so that it's accepted as input?

Forwards:
@SRR17088602.9 9/1
GNATCCTTGGATAAATTTATCTACTATTGGTTATAAAAAAGTTATGATAGGGAAATTAAAAAATATCGGATATTAATTGACACTTAAGAACTTCAAAGTGATGTGGCATCTCGAATTCAAATCCGTTTTGCTATATTAATAACTCGTTAG
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FF:FFFFFFFF::F,:F:
@SRR17088602.10 10/1
TNATTGTTTCCCCTCCAAGATTAGAAAAGAGAATAAAAAGACATGTTCCTTCTTCACCACCACTACTACCAAAAGAGAGTCAAGTATAAATTATTTTTTTTCAAAAATATTTTAATTACTTTTCTTAGCTATCTTCGCCACCAAGAATAG
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF,FFFFFFF::F::FFF:FFFFFFFFFFFFFFFFFFFFFFFFF:F:FFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFF

Reverse:
@SRR17088602.4 4/2
GTTATGATAGAAAAATTAAAAAATATCGGATATTAATTGACACTTAAGAACTTCACAGTGATGTGGCATCTCGAATTCAAATCCGTTTTGAAATATTAATAGCTCGTTAGAATCAGCATGAAAAACTGTATTAGAATCCTTGGATAAATT
+
F:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFF,FFFFFFFFFFFFFF,FFFFFFFFFF
@SRR17088602.5 5/2
GGAATGTTCTAAAGGATCTTTCAGTAACTGAATTTCCTTGAGACCAATGCGGTGTTGCATAGCTTTTGTTAATGTATAGCATTGAACAGAAATCCTTAAAGAACTCGCTTGTGAAAGCTGTGGCATTGTCAGTTACTAGCTCTACGCAGC
+
FFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

All the best.

[ndierckx]

Hi,

The reads you show here, are they each others pair, because the ids are not matching in any way..
And have you tried to run it already?

[lightworms]

Hello again!
Yes sorry I copy and pasted random sections from the forwards and reverse files in proper order it would look something like this:
Forwards:
@SRR17088602.1 1/1
ANAATTCTGGATCTCTAGGGGACACGGTATAAATTCTTCCGATACGAGGTTTACGATTTTTATTTTTCTCAGAAATATTTAACTTTTTCCAAACTTTATCATTTTTATTAAATGCATATGTTTCAGGAATATTTTCAAAAAGAATATTTT
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFF,FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@SRR17088602.2 2/1
TNTATATTTACACACCTGGAGGCAGTTTTCTAAAATTTTTGACTAATAAGGAAAATTCGAGAGGACCAAAAAAATTTTTTCTAAAGGACCCAAAAACATCGACAAAAATCCCCCAATTTTCCTAATTAGTCTTTTGATAATATTCGTAAT
+
F#FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Reverse:
@SRR17088602.1 1/2
TTTCAAATATGCATTTTTATCACATATAGTAATACCGTTAAGTATTCATTTACCAGACAACCAGAAAATTTATTTTACGAGTAAAAATGCAAGTAAAATTATTAGTGCTATTAATGCAGGTAAAATTCCAGATTCACGTCTTTCGGCTTA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFF
@SRR17088602.2 2/2
TGTGGAATAAGTTTTGAACAAAAATTGGGATTTTTTAAATTTGACAACGAATTTTTCATACAAAAATTTTTTGGAGGATGTAGAGAGACTACAAAAAAATTTATTCCACAATTTTTAAATAAATTACGAATATTATCAAAAGACTAATTA
+
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

I Did try to run it and got the following error (Log file is attached) : THE INPUT READS HAVE AN INCORRECT FILE FORMAT!
PLEASE SEND ME THE ID STRUCTURE!
log_SRR17088602_Arenaria_Test.txt

One way I could make it work though was by changing the format of the file from fastq to fasta.

All the best and thank you for taking the time to respond.