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Orientation

Estimation of the orientation of fibres in a volume

We estimate 3D cellular alignment based on image gradients and directional statistics.

The intensity gradients of the volumetric image are used to construct a 3D vector field and the local dominant orientations of this vector field then determined.

  • orientation_estimation performs the simulation and estimation of the orientation angles (requires cuda libraries)
  • generate_fibre_volume provides the FibreVolume class which produces the volume, positions the fibres within the volume and adds noise. Also provides additional functions to plot slices of the volume and histograms of the angles. The positioning of the fibres is a simple version based on the Quanfima package which provides a wider range of functionality than the one here
  • plot_orientation Produces 3D visualizations of the volumes using the VisPy library