DNA metabarcoding of gut contents, specifically COI gene on mitochondrial gene
https://rpubs.com/Scuba-Sarah/week_07
Zenodo For Week 09
Need to match sequences to known database from Smithsonian Institution
- Start with saw sequences
- Remove primer sequences
- Inspect read quality
- Filter and Trim
- Error Model
- Inference of true Biologial sequence variability (ASVs)
- Assign taxomony
- End point will be sequences with taxonomic ID, preferably down to species and with Lionfish reads removed
Week 5 Preliminary Results in output folder https://github.com/course-fish546-2023/Yerrace_metabarcoding/commit/1540cdf9a1662597664a40101f042c92743927c2