Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

ERROR: Paired-end reads were detected in single-end read library #45

Open
deb0612 opened this issue Oct 27, 2022 · 0 comments
Open

ERROR: Paired-end reads were detected in single-end read library #45

deb0612 opened this issue Oct 27, 2022 · 0 comments

Comments

@deb0612
Copy link

deb0612 commented Oct 27, 2022

Dear sir,
I tried to use fds<- countRNAData(fds) to count my bam file.
There are something wrong when running Subread:
ERROR: Paired-end reads were detected in single-end read library : /NGS_Storage/Debbie/Tumor/A3060/arriba/Aligned.sortedByCoord.out.bam
No counts were generated.
Error: BiocParallel errors
1 remote errors, element index: 1
0 unevaluated and other errors
first remote error:

image
Should I add any option? or I should direct use Subread?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant