All adjusted p-values at 1

[Cam-eraaa]

Dear FRASER2 team,

I am currently trying to use FRASER2 to analyse RNAseq data (mRNA and totalRNA), from muscle biopsy samples. I have been trying to reproduce the vignette on my data and I was surprised to see that all my adjusted p-values were all at 1. It seems that the vignette is also showing adjusted p-values at 1. I was wondering why this is, and how can this be resolved? The data has been tested already and we know that there are outliers in certain samples.

I am running the script on R 4.4.0 and the installed FRASER version is 2.0.0.

Thank you very much in advance!
Camille

[vyepez88]

Hi Camille, how many samples do you have? Usually we obtain pajusted values of 1 with <10 samples.