An Overview and Guide to Environmental DNA Protocols and Workflows

Orianne Tournayre (1, 2), Haolun (Allen) Tian (1), Stafford “Rotehrá:kwas” Maracle (1), David R. Lougheed (3), and Stephen C. Lougheed (1).

1. Department of Biology, Queen’s University, Kingston, Ontario, Canada
2. Current address. Department of Biology, York University, Toronto, Ontario, Canada
3. Canadian Centre for Computational Genomics, McGill University, Montreal, Quebec, Canada

Originally prepared for the Queen’s University Biological Station environmental DNA workshop

CC-BY-4.0 Creative Commons License 2022-2024

This has been a ‘living document’ that we have continued to edit & update each year and will continue to do so.

How to cite this document: Tournayre, O. Tian, H., Maracle, S., R., & Lougheed, S. C. (2024). An overview and guide to environmental DNA protocols and workflows. Version 2.2. Queen’s University, Kingston, Ontario, Canada. DOI: 10.5281/zenodo.13421371

Note: The DOI provided here links to all versions of the source code for this document. To access a specific version's DOI, see here or click "View all [n] versions" on the Zenodo page for a specific document version.

Project Structure

The manual is created using the Sphinx tool, and section text is written in the reStructuredText (RST) format (also see the Sphinx tutorial, which has some additional information on using RST).

The project is roughly structured as follows:

• Section files are stored in the ./src/sections folder.
• Images are stored in the ./src/images and should be high-resolution and, preferably, PNGs.
• Tables are stored either as images or as CSVs in ./src/tables folder, or alternatively are defined in-line in the section RST files.