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Automatic analysis of gene expression rate from time-course fluorescence assays data carried on microplate reader (bacterial cells)

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Gene-expression-rate-calculator

Author: Nicolas Kylilis PhD

Intended purpose:

Automated data analysis workflow for the calculation of gene expression rate

Suitable for time-course fluorescence assays on microplate-reader for bacterial cells

Experimental design (microplate layout):

Data analysis will only take place correctly if the following microplate layout is applied.

Column 1: Media blank

Column 2: Control cells for growth rate and autofluorescence

Column 3-12: Sample experimental conditions

Rows A-H: Replicates

Data analysis:

Open matlab software and run "growth_rate_module.m" file script. (Instructions for file naming etc can be found in the script)

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Automatic analysis of gene expression rate from time-course fluorescence assays data carried on microplate reader (bacterial cells)

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  • MATLAB 100.0%