Data and code for the paper

Yu, Y., Zeng, Z., Xie, D. et al. Interneuron origin and molecular diversity in the human fetal brain. Nat Neurosci (2021). https://doi.org/10.1038/s41593-021-00940-3

Introduction

There are two main parts of analysis of this work, one is single cell sequencing, another is in situ sequencing.

Single-cell sequencing

Following several scripts for the different parts of the analysis of single cell sequencing. Run them in this order:

1. Rscript R/Clustering of allsamples.r
2. Rscript R/progenitor_INPs_trajectory.r
3. Rscript R/progenitor_analysis.r
4. Rscript R/MGE_GW9_GW11.r
5. Rscript R/MGE_allweek.r
6. Rscript R/LGE_GW9_GW11.r
7. Rscript R/LGE_allweek.r
8. Rscript R/CGE_GW9_GW11.r
9. Rscript R/M1_analysis.r
10. Rscript R/Mapped_analysis.r

In situ sequencing

In situ sequencing are performed in three barin sections, and processed data are contained as follow:

1. ISS/GW9_CGE
2. ISS/GW12_MGE_LGE
3. ISS/GW12_CGE

We provide a web page for browse the distribution of single/multiple genes in sections performed by in situ sequencing (http://www.taolabissbrowser.site:3838/).