Upgrade htsjdk to v3.0.0.

build.gradle

@@ -60,8 +60,8 @@ repositories {
     mavenLocal()
 }
 
-final htsjdkVersion = System.getProperty('htsjdk.version','2.24.1')
-final picardVersion = System.getProperty('picard.version','2.27.1')
+final htsjdkVersion = System.getProperty('htsjdk.version','3.0.0')
+final picardVersion = System.getProperty('picard.version','2.27.4')
 final barclayVersion = System.getProperty('barclay.version','4.0.2')
 final sparkVersion = System.getProperty('spark.version', '2.4.5')
 final scalaVersion = System.getProperty('scala.version', '2.11')

src/main/java/org/broadinstitute/hellbender/engine/spark/GATKRegistrator.java

@@ -9,6 +9,7 @@
 import de.javakaffee.kryoserializers.guava.ImmutableMapSerializer;
 import htsjdk.samtools.*;
 import htsjdk.variant.variantcontext.Allele;
+import htsjdk.variant.variantcontext.SimpleAllele;
 import htsjdk.variant.vcf.VCFHeaderLineType;
 import htsjdk.variant.vcf.VCFInfoHeaderLine;
 import org.apache.spark.serializer.KryoRegistrator;
@@ -68,13 +69,13 @@ public VCFInfoHeaderLine newInstance() {
                 return new VCFInfoHeaderLine("TMP", 2, VCFHeaderLineType.String, "");
             }
         });
-        registration = kryo.register(Allele.class);
+        registration = kryo.register(SimpleAllele.class);
         registration.setInstantiator(new ObjectInstantiator<Allele>() {
             public Allele newInstance() {
                 return Allele.create("TCGA");
             }
         });
-    }
+   }
 
     @Override
     public void registerClasses(Kryo kryo) {

src/main/java/org/broadinstitute/hellbender/tools/funcotator/StrandCorrectedAllele.java

@@ -1,12 +1,13 @@
 package org.broadinstitute.hellbender.tools.funcotator;
 
 import htsjdk.tribble.annotation.Strand;
+import htsjdk.variant.variantcontext.SimpleAllele;
 
 /**
  * Class to represent a strand-corrected {@link htsjdk.variant.variantcontext.Allele}.
  * Created by jonn on 10/24/18.
  */
-public class StrandCorrectedAllele extends htsjdk.variant.variantcontext.Allele {
+public class StrandCorrectedAllele extends SimpleAllele {
     public static final long serialVersionUID = 1L;
 
     /**

src/main/java/org/broadinstitute/hellbender/tools/walkers/haplotypecaller/AssemblyBasedCallerUtils.java

@@ -338,11 +338,11 @@ public static AssemblyResultSet assembleReads(final AssemblyRegion region,
      * Handle pileup detected alternate alleles.
      */
     @VisibleForTesting
+    @SuppressWarnings("deprecation")
     static void processPileupAlleles(final AssemblyRegion region, final List<VariantContext> givenAlleles, final int maxMnpDistance,
                                      final int snpAdjacentToIndelLimit, final SmithWatermanAligner aligner, final Haplotype refHaplotype,
                                      final AssemblyResultSet assemblyResultSet, final int numHaplotypesPerIteration, final int hapFilteringKmerSize,
                                      final SWParameters haplotypeToReferenceSWParameters) {
-        final int assemblyRegionStart = region.getPaddedSpan().getStart();
         final int activeRegionStart = refHaplotype.getAlignmentStartHapwrtRef();
         final Map<Integer, VariantContext> assembledVariants = assemblyResultSet.getVariationEvents(maxMnpDistance).stream()
                 .collect(Collectors.groupingBy(VariantContext::getStart, Collectors.collectingAndThen(Collectors.toList(), AssemblyBasedCallerUtils::makeMergedVariantContext)));
@@ -419,10 +419,10 @@ public static void addGivenAlleles(final List<VariantContext> givenAlleles, fina
             final Allele longerRef = (assembledVC == null || givenVCRefLength > assembledVC.getReference().length()) ? givenVC.getReference() : assembledVC.getReference();
             final List<Allele> unassembledGivenAlleles = getAllelesNotPresentInAssembly(givenVC, assembledVC, givenVCRefLength, longerRef);
 
-            final List<Allele> unassembledNonSymbolicAlleles = unassembledGivenAlleles.stream().filter(a -> {
-                final byte[] bases = a.getBases();
-                return !(Allele.wouldBeNoCallAllele(bases) || Allele.wouldBeNullAllele(bases) || Allele.wouldBeStarAllele(bases) || Allele.wouldBeSymbolicAllele(bases));
-            }).collect(Collectors.toList());
+            final List<Allele> unassembledNonSymbolicAlleles = unassembledGivenAlleles.stream()
+                    //TODO, update the null allele check when htsjdk adds a NULL_ALLELE constant to Allele
+                    .filter(a -> !(a.equals(Allele.NO_CALL) || a.getDisplayString().equals(String.valueOf(VCFConstants.NULL_ALLELE)) || a.equals(Allele.SPAN_DEL) || a.isSymbolic()))
+                    .collect(Collectors.toList());
 
             // choose the highest-scoring haplotypes along with the reference for building force-calling haplotypes
             final List<Haplotype> baseHaplotypes = unassembledNonSymbolicAlleles.isEmpty() ? Collections.emptyList() : assembledHaplotypes.stream()

src/main/java/org/broadinstitute/hellbender/utils/haplotype/Haplotype.java

@@ -5,6 +5,7 @@
 import htsjdk.samtools.CigarOperator;
 import htsjdk.samtools.util.Locatable;
 import htsjdk.variant.variantcontext.Allele;
+import htsjdk.variant.variantcontext.SimpleAllele;
 import org.apache.commons.lang3.ArrayUtils;
 import org.apache.commons.lang3.tuple.Pair;
 import org.broadinstitute.hellbender.utils.SimpleInterval;
@@ -16,7 +17,7 @@
 import java.util.Arrays;
 import java.util.Comparator;
 
-public final class Haplotype extends Allele {
+public final class Haplotype extends SimpleAllele {
     private static final long serialVersionUID = 1L;
 
     /**