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• 👋 Hi, we're the @CCBR, a group of bioinformatics analysts and engineers
• 📖 We build flexible, reproducible, workflows for next-generation sequencing data
• 💡 We collaborate with CCR PIs
• 📫 You can reach us at [email protected]
• 🏁 Check out our release history
• 🔗 Our Zenodo community

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CCBR offers end-to-end analysis pipelines for NGS data analysis.

RHEL8 BIOWULF updates:

In late 2023, BIOWULF migrated to a new operating system, RHEL8. This migration rendered the Legacy functionality of CCBRPipeliner un-usable. We have been (and will continue to) work dilegently to bring ccbrpipeliner suite of pipelines back on-line for our Biowulf users. At the same time, we are also taking this opportunity to not only increase our repetoire of pipelines but also modernize and containerize our end-to-end analysis offerings. These changes will minimize, if not eliminate, the pipelines' dependencies on other Biowulf modules and make ccbrpipeliner "operating system and HPC" - agnostic, thereby making it shareable with collaborators, and runnable on other HPCs (like FRCE) and beyond.

Here is a list of our prominent pipelines and their release schedule on BIOWULF:

Data Type	Pipeline Name	CLI* availability date	GUI* availability date
RNASeq1	RENEE	July 3rd 2023	July 14th 2023
WESSeq2	XAVIER	July 21th 2023	Sep 1st 2023
ATACSeq3	ASPEN	November 30th 2023	TBD
ChIPSeq4	CHAMPAGNE	October 15th 2023	TBD
CRISPRSeq5	CRISPIN	September 31st 2023	TBD
CUT&RunSeq6	CARLISLE	October 31st 2023	TBD
EV-Seq10	ESCAPE	March 26th, 2024	TBD
circRNASeq7	CHARLIE	Jul 31st 2024	TBD
scRNASeq8	SINCLAIR	Sep 30th 2024	TBD
WGSSeq9	LOGAN	Sep 30th 2024	TBD
spatialSeq11	SPENCER	TBD	TBD

^{* CLI = Command Line Interface * GUI = Graphical User Interface}

^{1 RENEE=Rna sEquencing aNalysis pipElinE starts with raw fastq files and ends with counts matrix. Downstream DEG support will be added at a later date. In the mean time you can use NIDAP or iDEP for DEG analysis.}

^{2 XAVIER=eXome Analysis and Variant explorER will be soon available on Biowulf.}

^{3 ASPEN=Atac Seq PipEliNe has limited support for differential ATACSeq signal analysis. CCBR has other pipelines for footprinting analysis like TOBIAS. Please reach out for details.}

^{4 CHAMPAGNE=CHromAtin iMmuno PrecipitAtion sequencinG aNalysis pipEline. CCBR plans to completely revamp ChIPSeq and may not be available until Q4 of 2023. In the interim, we recommend using the ENCODE pipeline on biowulf for ChIPSeq analsyis.}

^{5 CRISPIN=CRISPr screen sequencing analysis pipelINe( previously called, CRUISE=Crispr scReen seqUencIng analySis pipEline ). CRISPRSeq analysis with MAGeCK, drugZ and BAGEL2.}

^{6 CARLISLE=Cut And Run anaLysIS pipeLinE supports human and mouse samples with (recommended) or without spike-ins.}

^{7 CHARLIE=Circrnas in Host And viRuses anaLysis pIpEline finds known and novel circRNAs in human/mouse + virus genomes. Differential circRNA analysis is planned for future.}

^{8 SINCLAIR=SINgle CelL AnalysIs Resource addresses various single cell modalities... eg. single-cell expression, CITESeq, TCR-Seq, etc.}

^{9 LOGAN=whoLe genOme-sequencinG Analysis pipeliNe will soon be CCBR's newest offering.}

^{10 ESCAPE=Extracellular veSiCles rnAseq PipelinE.}

^{11 SPENCER=SPatial seqeENCing Resource.}

For any other datatype or pipeline, please email 📫 us directly to get the conversation started!

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In additions to end-to-end analysis pipelines, the CCBR dev team also builds tools for data management, meta-data management, APIs, user management, etc. Here are some examples:

• spacesavers2
• permfix
• pyrkit

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module load ccbrpipeliner loads default release of ccbrpipeliner. Each release comprises of a unique combination of the version numbers of the different pipelines offered as part of the ccbrpipeliner suite.

^* = Current DEFAULT version on BIOWULF

^@ = CLI available

^# = GUI available

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Most of our end-to-end pipelines which have been used in published research work have been made available to the entire bioinformatics community via a Zenodo DOI. Please feel free to visit our Zenodo community page. And if you use our pipelines, don't forget to cite us!

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